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IJFS 2013, 12 Back to browse issues page
Isolation, identification and phylogenetic analysis of a pathogen of Haliotis diversicolor supertexta (L.) with mass mortalities
Z. Jing * , C. Junpeng
, scutqfnu@126.com
Abstract:   (6077 Views)
Studies were conducted to determine a disease outbreak in 14 day old post-larvae of abalone (Haliotis diversicolor supertexta) which caused mass mortality in July 2010 in Shanwei, China. Twenty-nine bacterial strains were isolated from a sample pool of 10 diseased post-larval abalones on 2216E marine agar plates during a natural outbreak of the disease. Among them, a dominant isolate (referred to as strain 21) was found to be highly virulent to post-larvae in experimental challenge tests, with an LD50 value of 1.0 ×104 colony forming units (CFU) mL-1 on day 3. API 20NE kits and 16S rDNA sequence analysis, identified strain 21 as Oceanomonas doudoroffii. It was susceptible to 10 and moderately susceptible to 1 of the 16 antibiotics examined when antibiotic sensitivities of the bacterium were assayed. Results of this study implicated Oceanomonas doudoroffii strain 21 as a cause of mortalities in post-larval abalone from Shanwei, China.
Keywords: Haliotis diversicolor supertexta, Massive death, Challenge test
Full-Text [PDF 144 kb]   (1935 Downloads)    
Type of Study: Orginal research papers | Subject: fish disease
Received: 2013/02/4 | Accepted: 2015/09/13 | Published: 2015/09/13
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Jing Z, Junpeng C. Isolation, identification and phylogenetic analysis of a pathogen of Haliotis diversicolor supertexta (L.) with mass mortalities. IJFS. 2013; 12 (1) :193-203
URL: http://jifro.ir/article-1-882-en.html


Volume 12, Issue 1 (1-2013) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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