[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
:: Volume 9, Issue 3 (9-2010) ::
IJFS 2010, 9 Back to browse issues page
Karyotype analysis in white bream (Blicca bjoerkna transcaucasica) from north coast of Iran
M. Pourkazemi * , S. Nazari , S. Bakhshalizadeh
, pkazemi_m@yahoo.com
Abstract:   (8628 Views)

  Preparation of chromosome spreads and karyotype analysis in Blicca bjoerkna transcaucasica were carried out using 0.01% solution of colchicines and Phytohaemagglutinin (PHA) (20 µg g-1 body weight). The gill and kidney tissues were collected and let to stand in a hypotonic solution of 0.075 M KCl and then treated with a fixative (Carnoy's solution) in three steps. The chromosomes spreads were then stained with 5% Giemsa solution for 20 min and examined under a light microscope. Appropriate metaphase plates were photographed in order to prepare karyotype. The size of the chromosomes (short and long arms), relative length of chromosomes and centromere index were calculated. Chromosome spreads from gill tissue cultures which were colchicine treated with PHA, had a well defined size, shape and number of chromosomes for karyotype analysis. Based on the 76 metaphase plates studied, chromosome count in 59 metaphase plates was 2n=49.74±0.25. By arranging homologous chromosomes beside each other the chromosome formula was calculated as 6 pairs of Metacentric, 10 pairs of Sub-Metacentric and 9 pairs of Sub-Telocentric (2n=6M+10+Sm+9St) and the chromosome arm number (NF) was 100. The largest chromosome in this species was a pair of metacentric chromosomes. On the basis of the number and type of chromosomes, the karyotype obtained for this species conformed to the findings of other researchers, but the chromosome formula was different, which could be attributed to the existence of different populations for this species.

Keywords: Blicca bjoerkna transcaucasica, Phytohaemagglutinin, Colchicines, Karyotype
Full-Text [PDF 317 kb]   (2640 Downloads)    
Type of Study: Orginal research papers | Subject: Genetics
Received: 2010/09/18 | Accepted: 2015/09/13 | Published: 2015/09/13
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Pourkazemi M, Nazari S, Bakhshalizadeh S. Karyotype analysis in white bream (Blicca bjoerkna transcaucasica) from north coast of Iran. IJFS. 2010; 9 (3) :454-463
URL: http://jifro.ir/article-1-73-en.html


Volume 9, Issue 3 (9-2010) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 3705