The present study was aimed to evaluate the antimicrobial resistance and the presence of antibiotic resistance genes in Vibrios spp. isolated from seafood. A total of 72 isolates of Vibrio in 6 species including V. parahaemolyticus, V. vulnificus, V. alginolyticus, V. harveyi, V. mimicus and V. cholerae were examined. The results revealed that all isolates were expressing multiple antibiotic resistances. Of the 72 strains tested, 70 were resistant to ampicillin (97.2%), 60 to gentamycin (83.3%) and 56 to penicillin (77.7%). Eight strains were resistant to 4 antibiotic, 19 resistant to five antibiotics, 10 to six antibiotics, 34 to seven antibiotics and one to eight antibiotics. Results also revealed that 20 Vibrio strains (27.7% of total examined strains) contained one to three of the antibiotic resistance genes. StrB, tetS and ermB genes coding for streptomycin, tetracycline and erythromycin resistance were found in 18, 6, 5 isolates, respectively and Sulfamethoxazole resistance gene, sul2, was not detected in this study. Detection of resistance genes in Vibrio strains obtained from seafood is considered as a potential danger for consumers and also suggests that these resistance determinants might be further disseminated in habitats, thus constituting a serious health risks to human.