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:: دوره 21، شماره 5 - ( 1401 ) ::
جلد 21 شماره 5 صفحات 1191-1180 برگشت به فهرست نسخه ها
Research Article: Bioactive peptides from the Pacific white-leg shrimp (Litopenaeus vannamei) induce apoptosis and anticancer activities in HCT-116 colon cancer cell line
چکیده:   (909 مشاهده)
In this study, the effects of white-leg shrimp hydrolysate protein extracted in a progressive rise in temperature (40-60°C) (Gradual) and without centrifugation (Mix) on the colon cancer cells were determined. Both bioactive peptides were prepared using enzymatic hydrolysis with alcalase. The cytotoxic effect on HCT-116 cell line was evaluated using the Neutral red and MTT assay. In-vitro, antioxidant activity was performed using DPPH, TAC, and nitric oxide assays. Apoptosis with acridine orange/etho bromide and redox changes was evaluated in the cell lines.The results of toxicity assays showed that the survival rate of the cells were decreased by increasing the concentrations of the peptides (0.3, 0.6, 1.2, and 2.4 mg/mL). Based on the results of antioxidant activity (TAC, DPPH, No), the Gradual peptides had significantly higher antioxidant activity than Mix peptides (p<0.05). In addition, the Gradual peptide increased the concentration of nitric oxide compared to the control and Mix groups (p<0.05). Results also showed that the Gradual peptides reduced the secretion of catalase and GSH enzymes in the cancer cells (p<0.05). Both types of peptides increased apoptosis in the cancerous cell line and a higher value was observed in bioactive peptides treatments The results of the present study showed that the Pacific white shrimp hydrolysates protein obtained in a progressive rise in temperature showed anti-cancer activities against colon HCT-116cancer cell line.
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نوع مطالعه: پژوهشي | موضوع مقاله: Biology & physiology
دریافت: 1400/3/12 | پذیرش: 1401/3/8 | انتشار: 1401/9/27
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Azadi N, H. Najdegerami E, Imani M, Nikoo M. Research Article: Bioactive peptides from the Pacific white-leg shrimp (Litopenaeus vannamei) induce apoptosis and anticancer activities in HCT-116 colon cancer cell line. IJFS 2022; 21 (5) :1180-1191
URL: http://jifro.ir/article-1-4743-fa.html

Research Article: Bioactive peptides from the Pacific white-leg shrimp (Litopenaeus vannamei) induce apoptosis and anticancer activities in HCT-116 colon cancer cell line. مجله علوم شیلاتی ایران. 1401; 21 (5) :1180-1191

URL: http://jifro.ir/article-1-4743-fa.html



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دوره 21، شماره 5 - ( 1401 ) برگشت به فهرست نسخه ها
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
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