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:: دوره 11، شماره 1 - ( 1390 ) ::
جلد 11 شماره 1 صفحات 145-155 برگشت به فهرست نسخه ها
Titration of the Iranian White Spot Virus isolate, on Crayfish Astacus leptodactylus and Penaeus semisulcatus
چکیده:   (9270 مشاهده)

  White Spot Virus (WSV) is currently the most serious viral pathogen of shrimp worldwide it causes mortality up to 100% within 7-10 days in commercial shrimp farms. Infected Indian white shrimp Fenneropenaeus indicus samples were collected from Guatr shrimp site in Sistan and Baluchestan province in south of Iran and WSV infection was confirmed by Nested PCR. WSV was isolated from infected shrimp samples by centrifugation and filtration and multiplied in crayfish by intramuscular inoculation, the isolated virus was called WSV/IRN/1/2010. In order to determine the dilution resulting in 90-100% mortality in Penaeus semiculcatus, diluted virus stock in steps from 100 till 105 times in sterile PBS was injected intramuscularly to 14 shrimps in each group. Also the virus stock was diluted in steps from 1/2 till 1/32 times in sterile PBS and injected intramuscularly in Astacus leptodactylus crayfish. Therefore the LD50 of live virus stock in Astacus leptodactylus and Penaeus semiculcatus crayfish were calculated by the Karber method 10 3.29 /ml and 10 5.35 /ml, respectively.

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نوع مطالعه: پژوهشي | موضوع مقاله: fish disease
دریافت: 1390/12/27 | پذیرش: 1394/6/22 | انتشار: 1394/6/22
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Motamedi Sedeh F, Afsharnasab M, Heidarieh M, Shafaee S K, Rajabifar S, Dashtiannasab A et al . Titration of the Iranian White Spot Virus isolate, on Crayfish Astacus leptodactylus and Penaeus semisulcatus. IJFS. 2012; 11 (1) :145-155
URL: http://jifro.ir/article-1-450-fa.html

Titration of the Iranian White Spot Virus isolate, on Crayfish Astacus leptodactylus and Penaeus semisulcatus. مجله علوم شیلاتی ایران. 1390; 11 (1) :145-155

URL: http://jifro.ir/article-1-450-fa.html



دوره 11، شماره 1 - ( 1390 ) برگشت به فهرست نسخه ها
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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