[صفحه اصلی ]   [Archive]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 19، شماره 5 - ( 1399 ) ::
جلد 19 شماره 5 صفحات 2384-2393 برگشت به فهرست نسخه ها
Isolation and identification of antibacterial steroid compounds from Ulva fasciata in the Persian Gulf
چکیده:   (73 مشاهده)
Due to numerous reasons, marine-obtained resources have attracted the interest of researchers. One of the subjects attracted the interest of researchers in recent years in the field of biologist marine algae due to their nutritional value, their benefits for health and their biological activities. In this study, the powder was extracted using acetone, then the extract purified by silica gel column chromatography with n-hexane and ethyl acetate combination to isolate the steroid compounds. Isolated compound was run through TLC and sprayed with vanillin-sulphuric acid reagent to detect the steroid compounds and profile of isolated compounds obtained by gas chromatography (GC). Antibacterial activity of steroids extracted of green algae Ulva fasciata from Qeshm Island in the Persian Gulf were assessed (in vitro). The antibacterial activity of the fractions was determined by Broth Dilution Methods against clinical Gram-negative bacteria: Escherichia coli, Pseudomonas aeruginosa, Salmonella. typhi and Gram-positive bacteria: Staphylococcus aureus, Bacillus subtilis, Bacillus cereus. The steroids; cholest-7-en-3ol and cholestan-3-one, cyclic 1, 2-ethanediyl acetal compounds in column fraction were identified by GC. The results showed the minimum bactericidal concentration (MBC) of the cholest-7-en-3ol and cholestan-3-one, cyclic 1,2-ethanediyl acetal for S. aureus and Bacillus cereus were 500 μg ml-1, the MBC of the cholest-7-en-3ol for B. subtilis was 500 μg ml-1 and 1000 μg ml-1 detected for cholestan-3-one, cyclic 1,2-ethanediyl acetal. The compounds had neither antibacterial activity on gram negative bacteria. Based on our results, extracted steroids from U. fasciata can be considered as a source of novel antibiotics.
متن کامل [PDF 295 kb]   (58 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: Biotechnology
دریافت: 1398/8/25 | پذیرش: 1398/10/7 | انتشار: 1399/6/30
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA


XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Azizi R, Motallebi Moghanjoughi A, Nazemi M, Sharif Ruhani M, Afshar Nasab M. Isolation and identification of antibacterial steroid compounds from Ulva fasciata in the Persian Gulf. IJFS. 2020; 19 (5) :2384-2393
URL: http://jifro.ir/article-1-4116-fa.html

Isolation and identification of antibacterial steroid compounds from Ulva fasciata in the Persian Gulf. مجله علوم شیلاتی ایران. 1399; 19 (5) :2384-2393

URL: http://jifro.ir/article-1-4116-fa.html



دوره 19، شماره 5 - ( 1399 ) برگشت به فهرست نسخه ها
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4219