[صفحه اصلی ]   [Archive]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 19، شماره 6 - ( 1399 ) ::
جلد 19 شماره 6 صفحات 2824-2845 برگشت به فهرست نسخه ها
Research Article: Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila
چکیده:   (535 مشاهده)
Our study was focused on characterization of dominant Bacillus bacteria isolated from the digestive tract of Oncorhynchus mykiss. After 8 weeks, the fish fed with probiotic-free diet were dissected under strictly controlled conditions. Fish intestines were eviscerated and its content diluted to 10−4 using Ringer solution. Two isolated bacteria were Gram-positive, catalase-positive, and oxidase-negative, based on the biochemical tests. A mixed of the two strains was the most efficient isolates determined in O. mykiss. Molecular characterization using PCR-Ribotyping was carried out using sequencing the 16S rRNA gene of both strains, which isolated two bacteria, Bacillus subtilis Ahari.H1 and B. licheniformis Ahari. H2. The isolated bacteria could tolerate 0.3% bile salt and non-hemolytic in nature. They also showed resistance to pH at 2.5 and 4 for acid test and 2.3 and 7 for gastric juice, pepsin and trypsin. It is concluded that the efficiency of the isolated probiotics can be candidates as probiotic in fish culture. It was unveiled that these strains can successfully diminish Aeromonas hydrophila pathogenicity in rainbow trout.
 
متن کامل [PDF 874 kb]   (220 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: aquaculture
دریافت: 1398/6/12 | پذیرش: 1399/3/13 | انتشار: 1399/8/30
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA


XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ebne al-Torab S, Ahari H, Kakoolaki S. Research Article: Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila. IJFS. 2020; 19 (6) :2824-2845
URL: http://jifro.ir/article-1-4043-fa.html

Research Article: Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila. مجله علوم شیلاتی ایران. 1399; 19 (6) :2824-2845

URL: http://jifro.ir/article-1-4043-fa.html



دوره 19، شماره 6 - ( 1399 ) برگشت به فهرست نسخه ها
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4274