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Determination of the optimal conditions of cloning Aerolysin gene from the common carp pathogen Aeromonas hydrophila in Escherichia coli BL21
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I.R. Ibrahim *   , S.M.A. Abdullah , A.Y. Abdulkarim Yasin Karim |
| Fish Recourses and Aquatic Animals Department, College of Agriculture Salahaddin University-Erbil, Iraq. , r_83_ibrahem@yahoo.com |
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Abstract: (2461 Views) |
| Aeromonas hydrophila is a gram-negative bacterium which associated with gastrointestinal diseases and septicaemia. This pathogenic bacterium has several virulence factors ranging from pili to the excreted protein which called (Aerolysin) with minor and major effects, respectively. Additionally, Aeromonas hydrophila is a widely distributed bacterium that commonly causes ulcers in cyprinid fish such as carps and secondary diseases in humans as well. In the present study, characteristics and haemolytic activities of the recombinant Aerolysin protein and optimal conditions for cloning are determined using the synthesized cloning/expression Aerolysin gene, assembled into the Escherichia coli BL21 (DE3) through pGEX-6P1 vector, using SDS-PAGE and western blotting techniques. The results declared that, the Aerolysin gene (1482 bp) was cloned by transforming the recombinant pGEX-6P1 vector into Escherichia coli BL21 (DE3) as a prokaryotic expression host. The SDS-PAGE results indicated that the estimated protein size was 54 KDa. Recombinant Aerolysin protein synthesis at both selected temperatures, 25°C and 37°C, indicated that 1 mM of isopropyl-β-D-thiogalactopyranoside (IPTG) was the optimum concentration for induction. However, the recombinant protein was unable to synthesize in the absence of IPTG inducer. Western blot analysis indicated the efficient sensitivity and specificity of the recombinant Aerolysin protein. In conclusion, the recombinant protein showed potential advantages for immunoassay approaches in order to decrease the economic losses caused by disease in the aquaculture industry. |
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| Keywords: Aeromonas hydrophila, Escherichia coli common Carp, Fish disease, Haemolytic activity, Recombinant protein, Aerolysin |
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Full-Text [PDF 746 kb]
(1535 Downloads)
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Type of Study: Orginal research papers |
Subject:
aquaculture
Received: 2019/05/27 | Accepted: 2019/07/10 | Published: 2020/09/20
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