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Some effects of experimental acidification on phenoloxidase, trypsin and lysozyme activities in freshwater crayfish (Pontastacus leptodactylus)
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M. Soltani *   , S. Khazraeenia , H. Sepehri |
| , msoltani@ut.ac.ir |
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Abstract: (3724 Views) |
| Levels of phenoloxidase (Po), trypsin and lysozyme activities were determined following 48 hours exposing freshwater crayfish (Pontastacus leptodactylus) weighing 59.8±3.4g to different low pH values of 6.5, 5.5, 4 and 2.5 at 20-23˚C. Significant decrease were found (p<0.01) in levels of Po and trypsin activities in crayfish haemocyte lysate supernatant (HLS) at pHs 5.5 and 4 compared to the control group (pH 8). Also, plasma lysozyme activity showed a significant increase in water with pHs of 6.5, 5.5 and 4 (p<0.01). Furthermore, measurement of calcium and magnesium concentrations in sera samples showed a significant decrease for calcium ion at pHs 5.5 and 4, while magnesium concentration was significantly decreased only at pH 4 compared to control group (p<0.05). Crayfish exposed to pH 2.5 died within 24 hours post-exposure. |
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| Keywords: Crayfish, Pontastacus leptodactylus, phenoloxidase, trypsin, lysozyme, serum calcium and magnesium |
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Full-Text [PDF 2657 kb]
(1334 Downloads)
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Type of Study: Orginal research papers |
Subject:
fish disease
Received: 2017/12/5 | Accepted: 2017/12/5 | Published: 2017/12/5
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