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IJFS 2008, 7 Back to browse issues page
Some effects of experimental acidification on phenoloxidase, trypsin and lysozyme activities in freshwater crayfish (Pontastacus leptodactylus)
M. Soltani * , S. Khazraeenia , H. Sepehri
, msoltani@ut.ac.ir
Abstract:   (497 Views)
Levels of phenoloxidase (Po), trypsin and lysozyme activities were determined following 48 hours exposing freshwater crayfish (Pontastacus leptodactylus) weighing 59.8±3.4g to different low pH values of 6.5, 5.5, 4 and 2.5 at 20-23˚C. Significant decrease were found (p<0.01) in levels of Po and trypsin activities in crayfish haemocyte lysate supernatant (HLS) at pHs 5.5 and 4 compared to the control group (pH 8). Also, plasma lysozyme activity showed a significant increase in water with pHs of 6.5, 5.5 and 4 (p<0.01). Furthermore, measurement of calcium and magnesium concentrations in sera samples showed a significant decrease for calcium ion at pHs 5.5 and 4, while magnesium concentration was significantly decreased only at pH 4 compared to control group (p<0.05). Crayfish exposed to pH 2.5 died within 24 hours post-exposure.
Keywords: Crayfish, Pontastacus leptodactylus, phenoloxidase, trypsin, lysozyme, serum calcium and magnesium
Full-Text [PDF 2657 kb]   (212 Downloads)    
Type of Study: Orginal research papers | Subject: fish disease
Received: 2017/12/5 | Accepted: 2017/12/5 | Published: 2017/12/5
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Soltani M, Khazraeenia S, Sepehri H. Some effects of experimental acidification on phenoloxidase, trypsin and lysozyme activities in freshwater crayfish (Pontastacus leptodactylus) . IJFS. 2008; 7 (1) :87-102
URL: http://jifro.ir/article-1-3084-en.html


Volume 7, Issue 1 (1-2008) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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