Persian bee propolis and pollen extracts enhanced the non-specific immune response of rainbow trout (Onchorhynchus mykiss) and resistance against Aeromonas hydrophila
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N. Choobkar * |
, nchoobkar20@iauksh.ac.ir |
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Abstract: (4598 Views) |
The effect of Persian bee propolis and pollen extracts on innate immune response of rainbow trout (Onchorhynchus mykiss) was investigated. Fish were fed diets containing 1% Pollen, 1% propolis and combination of the two immune-stimulants (0.5% Pollen and 0.5% propolis) as treatment groups and fed without the two immune-stimulants as positive and negative control groups for 4 weeks. The effects of different stimulants at different weeks (Time × stimulants) were analyzed with the Repeated Measures ANOVA. The first-four groups were then subjected to Aeromonas hydrophila for 5 days. The phagocytic activity, respiratory burst activity, Plasma Total Protein and cumulative mortality were monitored. The results showed that fish fed diet containing 1% propolos or 0.5% Pollen+ 0.5% propolis enhanced non-specific immunity including respiratory burst activity, phagocytosis but decreases the mortality in challenging with A. hydrophila by 45 and 65%, respectively. |
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Keywords: Propolis, Pollen, Non-specific immunity, Onchorhynchus mykiss |
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Received: 2015/09/7 | Accepted: 2016/04/7 | Published: 2017/06/7
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