[Home ]      
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
:: Volume 16, Number 1 (2-2017) ::
Back to browse issues page IJFS 2017, 16
Study of genetic diversity of wild Caspian trout Salmo trutta caspius in the Sardabrud and Astara Rivers, using D- Loop region sequencing
S. Najjar Lashgari *, S. Rezvni Gilkolaei, A. Kamali, M. Soltani
, se_lashgari@yahoo.com
Abstract:   (131 Views)

In this study the genetic diversity of wild Caspian trout (Salmo trutta caspius) in the Sardabroud and Astara Rivers was evaluated using D- Loop region sequencing. For this purpose, 35 specimens of adult Caspian brown trout were collected from these rivers in the Mazandarn and Gilan Provinces in fall and winter 2011. Approximately 3-5 g of soft and fresh fin tissue was isolated and fixed in ethanol 96% and then transferred to the Caspian Sea Ecology Research Center Genetics Laboratory in Sari, Iran. Genomic DNA from the samples was extracted using Ammonium Acetate Method. The quality and quantity of the extracted DNA were assessed by spectrophotometer and agarose gel (1%) electrophoresis. Polymerase Chain Reaction (PCR) was performed on the target DNA using a primers sequence D- Loop region of mtDNA molecule. Then the product was purified and DNA sequencing was carried out using chain termination method. The D- Loop region of Caspian trout contained 654 bp. Data were analyzed using Bio-Edit, DnaSP, Arlequin and Mega software. 20 and 15 haplotypes was observed in Sardabrud and Astara River. Tthe DNA sequence of one of them was recorded in Gene Bank with numbers KC991027 and KF015727. 223 and 240 polymorphic loci were detected in Sardabrud and Astara River that all of them were out of Hardy- Weinberg equilibrium (p<0.05). Average nucleotide and haplotype diversity were 0.127±0.067, 1.000±0.005 in Sardabrud River and 0.118±0.063 and 1.000±0.005 in Astara River.

Keywords: Genetic diversity, Salmo trutta caspius, Sardabrud, Astara, Sequencing
Full-Text [PDF 308 kb]   (79 Downloads)    
Type of Study: Orginal research papers | Subject: aquaculture
Received: 2017/02/21 | Accepted: 2017/02/21 | Published: 2017/02/21
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Najjar Lashgari S, Rezvni Gilkolaei S, Kamali A, Soltani M. Study of genetic diversity of wild Caspian trout Salmo trutta caspius in the Sardabrud and Astara Rivers, using D- Loop region sequencing . IJFS. 2017; 16 (1) :352-365
URL: http://jifro.ir/article-1-2602-en.html
Back to browse issues page Volume 16, Number 1 (2-2017)
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.045 seconds with 789 queries by yektaweb 3302