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The determination of different effective concentration of ethanolic extract of bee pollen on biochemical analysis in liver, spleen and heart tissues of rainbow trout, Oncorhynchus mykiss (Walbaum, 1792)
S.D. Dastan, M.F. Gulhan, Z. Selamoglu *, T. Dastan
, zselamoglu@ohu.edu.tr
Abstract:   (527 Views)

The aim of this study was to investigate the effective concentration of ethanolic extract  of bee pollen on liver, spleen and heart tissues of fish. Bee pollen extract in various concentrations (0.5, 2.5, 5, 10, 20 and 30 ppm) was administered to fish for 96 h under aquarium conditions. The malondialdehyde (MDA) levels, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) and levels of total free sulfhydryl groups were investigated in liver, spleen and heart in fish samples. MDA levels in liver, spleen and heart tissues of various concentration groups (2.5, 5, 10, 20 and 30 ppm) decreased (p<0.05) compared to the control group. The highest value of TAS (P<0.05) and the lowest value of TOS (p<0.05) occured in liver and heart tissues of 10 and 20 ppm concentration groups. The lowest levels of OSI were recorded in liver and heart tissues of 10, 20 and 30 ppm concentration groups compared to control group (p<0.05). The highest values of total sulfhydryl groups were recorded (p<0.05) in all tissues of 10 and 20 ppm groups compared with that in the control group. Finally it was observed that in liver, spleen and heart tissues of fish, the antioxidant effects of ethanolic extract of bee pollen depended on concentrations.

Keywords: Pollen extract, Malondialdehyde, Antioxidant status, Oxidant status, Free sulfhydryl, Oncorhynchus mykiss
Full-Text [PDF 278 kb]   (315 Downloads)    
Type of Study: Orginal research papers | Subject: Biotechnology
Received: 2017/02/19 | Accepted: 2017/02/19 | Published: 2017/02/19
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Dastan S, Gulhan M, Selamoglu Z, Dastan T. The determination of different effective concentration of ethanolic extract of bee pollen on biochemical analysis in liver, spleen and heart tissues of rainbow trout, Oncorhynchus mykiss (Walbaum, 1792). IJFS. 2017; 16 (1) :326-340
URL: http://jifro.ir/article-1-2600-en.html
Back to browse issues page Volume 16, Number 1 (1-2017)
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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