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:: دوره 19، شماره 2 - ( 1398 ) ::
جلد 19 شماره 2 صفحات 768-779 برگشت به فهرست نسخه ها
Isolation and characterization of acid-soluble collagen from the skin of Rutilus frisii kutum (Kamensky) of the Caspian Sea
چکیده:   (906 مشاهده)
This study proceeds to extract collagen from the skin of Rutilus  kutum, one of  the main and economical species of the Caspian Sea. Acid-soluble collagen (ASC) from the skin of R. kutum was extracted and characterized by SDS-PAGE, FTIR and UV spectrophotometry. Based on the data from the SDS-PAGE and the composition of amino acid, it was probable that the obtained collagen classified as type I collagen. Collagen consists of two different types of α chains, including α1 and α2, and β components. Also, FTIR analysis revealed the presence of the helical arrangements of the collagen. Moreover, UV spectrophotometry exhibited this collagen has an absorbance at 220 nm. On the basis of dry weight, the yield of ASC was calculated as 15.6%. The amount of proline in ASC was 89.6 residues per 706.1 residues, and 25.7% of the collagen was composed of glycine. These results showed that R. Kutum skin could be considered as a possible source of collagen and can supply the theoretical basis for further research.
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نوع مطالعه: پژوهشي | موضوع مقاله: Biotechnology
دریافت: 1395/11/3 | پذیرش: 1396/1/23 | انتشار: 1399/1/1
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Naderi Gharagheshlagh S, Fatemi M, Jamili S, Nourani A, Nourani M. Isolation and characterization of acid-soluble collagen from the skin of Rutilus frisii kutum (Kamensky) of the Caspian Sea. IJFS. 2020; 19 (2) :768-779
URL: http://jifro.ir/article-1-2543-fa.html

Isolation and characterization of acid-soluble collagen from the skin of Rutilus frisii kutum (Kamensky) of the Caspian Sea. مجله علوم شیلاتی ایران. 1398; 19 (2) :768-779

URL: http://jifro.ir/article-1-2543-fa.html



دوره 19، شماره 2 - ( 1398 ) برگشت به فهرست نسخه ها
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
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