:: Volume 18, Issue 4 (2019) ::
IJFS 2019, 18 Back to browse issues page
Effect of enviromental temperature on heat shock proteins (HSP30, HSP70, HSP90) and IGF-I mRNA expression in Sparus aurata
A. Bildik * , G.S. Asıcı Ekren , G. Akdeniz , F. Kıral
Adnan Menderes University, Veterinary Medicine Faculty, Biochemistry Department, Aydın/TURKEY , bildik65@hotmail.com
Abstract:   (3041 Views)

Ambient temperature is one of the most important environmental factors affecting physiological mechanisms and biochemical reactions of living organisms. Thus the effect of ambient temperature on HSPs and IGF-I gene expression levels in the liver and muscle tissues of Sparus aurata were investigated in this research. The levels of HSPs, and IGF-I gene expression of the liver and muscle of Sparus aurata were analyzed in by qRT-PCR. The experiment was done   in July (27 C) and  January (18C). HSP70 mRNA relative expression levels in the muscle on January were significantly higher than July (approximately 1.7 fold), whereas HSP30 gene expression in the liver on July was increased by 2.0 fold (p<0.05). Transcription of other heat shock proteins and IGF-I were not affected by water temperature changing. The HSP findings of the research show that these proteins are important and sensitive in the average adaptation.

Keywords: Sparus aurata, Temperature, IGF-I, HSPs, Gene expression
Full-Text [PDF 357 kb]   (2093 Downloads)    
Type of Study: Orginal research papers | Subject: Biochemistry
Received: 2016/10/3 | Accepted: 2017/04/8 | Published: 2019/09/19


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Volume 18, Issue 4 (2019) Back to browse issues page