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:: Volume 15, Issue 2 (4-2016) ::
IJFS 2016, 15 Back to browse issues page
Evaluation of acetylcholinesterase transcript level as a biomarker of methylmercury in orange spotted grouper (Epinephelus coioides) brain
Sh. Savari * , A.R. Safahieh , B. Bita Archangi , A. Savari , R. Abdi
, srsavari@yahoo.com
Abstract:   (2394 Views)

The bioavailability of methylmercury (MeHg) in the brains of  orange spotted groupers, captured from four creeks of Mahshahr embayment was measured.  Then the effects of this pollutant on the regulation of gene expression, acetylcholinesterase transcript levels was chosen in order to monitor the amounts of methylmercury concentrations in the creeks, and the fluctuations of mRNA expressions in the brain and their effect on fish health. Fishes were collected from Zangi, Ghanam, Marymous and Petrochemical Creeks, and their brains were removed by dissection. In parallel with these experiments some fishes were exposed to methylmerucry chloride in the Fisheries center and the amount of their gene expression was assessed via Real-Time PCR method. The lethal concentration of methylmerucry causing the mortality of half of the fish population after 96 hr (LC50-96) was assessed and gene expression of sub-lethal concentration (more and less than 10% of LC50-96) were analyzed. Gene expression studies revealed that the most polluted creek was the Petrochemical Creek, and the least polluted one was Marymous Creek. This regulation was assessed by the effect of MeHg on the gene expression, meaning the more gene expression, the less polluted and vice versa. From this study we concluded that acetylcholinesterase gene expression can serve as a biomarker of the effect of methylmercury, which can provide a good estimation of the amount of methylmercuric availability in the brain of Epinephelus coioides and its effect on the brain neurotransmission pathway.  

Keywords: Acetylcholinesterase (AChE), Methylmercury (MeHg), Epinephelus coioides, Orange spotted grouper, Transcript level, Brain
Full-Text [PDF 431 kb]   (873 Downloads)    
Type of Study: Orginal research papers | Subject: Biology & physiology
Received: 2016/05/16 | Accepted: 2016/05/16 | Published: 2016/05/16
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Savari S, Safahieh A, Bita Archangi B, Savari A, Abdi R. Evaluation of acetylcholinesterase transcript level as a biomarker of methylmercury in orange spotted grouper (Epinephelus coioides) brain. IJFS. 2016; 15 (2) :898-912
URL: http://jifro.ir/article-1-2242-en.html


Volume 15, Issue 2 (4-2016) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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