[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
:: Volume 15, Issue 2 (4-2016) ::
IJFS 2016, 15 Back to browse issues page
Molecular identification of some causative agents of warm-water streptococcosis by M-PCR in cultured rainbow trout, Chaharmahal - Bakhtiari Province, Iran
M. Raissy * , M. Sarshoughi , M. Moumeni
, mehdi.raissy@iaushk.ac.ir
Abstract:   (2298 Views)

Streptococcosis has been defined as a hemorrhagic septicemia in many fish species especially rainbow trout. This disease causes serious economic losses due to high mortality in fish and the bacteria responsible is also considered as a zoonotic pathogen. The aim of this study was identification of different causative agents of warm water streptococcosis and evaluation of the capsule gene in Lactococcus garvieae isolates collected from rainbow trout in Chaharmahal - Bakhtiari Province, Iran. A total of 430 clinically suspected fish were collected from 43 rainbow trout farms. The samples were analyzed using biochemical methods and multiplex-PCR. The results indicated that samples of 36 fish farms (83.7%) were infected with one of the studied bacteria. The infection by L. garvieae was found to be more than that by other species (30 of 36 farms, 83%). Infection by S. iniae and S. uberis was found in nine and one fish farm, respectively. Presence of virulence genes was studied in L. garvieae isolates by PCR assay. According to the results 34 of 50 isolates (68%) contained the capsule gene. The results revealed high prevalence of virulent L. garvieae in rainbow trout farms which should be considered in preventional policies such as vaccine production and also in public health. 

Keywords: Streptococcosis, Virulence gene, Rainbow trout, PCR, Iran
Full-Text [PDF 362 kb]   (1409 Downloads)    
Type of Study: Orginal research papers | Subject: fish disease
Received: 2016/05/7 | Accepted: 2016/05/7 | Published: 2016/05/7
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Raissy M, Sarshoughi M, Moumeni M. Molecular identification of some causative agents of warm-water streptococcosis by M-PCR in cultured rainbow trout, Chaharmahal - Bakhtiari Province, Iran. IJFS. 2016; 15 (2) :836-845
URL: http://jifro.ir/article-1-2224-en.html


Volume 15, Issue 2 (4-2016) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.06 seconds with 31 queries by YEKTAWEB 3742