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IJFS 2016, 15 Back to browse issues page
Isolation and screening of antibacterial and enzyme producing marine actinobacteria to approach probiotics against some pathogenic vibrios in shrimp Litopenaeus vannamei
M. Gozari * , M.S. Mortazavi , N. Bahador , S. Tamadoni jahromi , M. Rabbaniha
, m_gozari@yahoo.com
Abstract:   (3115 Views)

The application of new probiotics is a good strategy in the biological control of infectious diseases in aquaculture. Approximately 100 marine actinobacteria isolates were obtained from 10 sediment samples of shrimp farms. Heat treatment of sediment samples resulted in a selective reduction of the non actinobacterial heterotrophic microflora. Starch nitrate agar medium exhibited more efficacy than glycerol arginine agar medium for isolation. Twenty seven percent of actinobacterial isolates showed antagonistic activities against pathogenic Vibrio spp. All the antagonistic isolates showed the typical morphology of genus Streptomyces. Exoenzymatic activity screening showed that 44 %, 26%, 37% of antagonistic isolates represented amylase, lipase and protease activities, respectively. MNM-1400 strain exhibited highest antagonistic and exoenzymatic activity. The pathogenicity experiment revealed that MNM-1400 strain did not cause disease in Litopenaeus vannamei larvae. Extraction of produced antibacterial compounds by MNM-1400 strain showed that the active constituent didn’t have non polar property. Morphological, physiological and biochemical identification confirmed that MNM-1400 strain belonged to the genus streptomyces. Phylogenetic analysis by 16S rRNA gene sequencing showed a high similarity between MNM-1400 strain and Streptomyces californicus (similarity: 99%). These results suggest that the MNM-1400 strain can be considered as a potential probiotic in aquaculture

Keywords: Probiotic, Marine actinobacteria, Biological control, Shrimp, Litopenaeus vannamei
Full-Text [PDF 330 kb]   (1643 Downloads)    
Type of Study: Orginal research papers | Subject: Biotechnology
Received: 2016/04/23 | Accepted: 2016/04/23 | Published: 2016/04/23
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Gozari M, Mortazavi M, Bahador N, Tamadoni jahromi S, Rabbaniha M. Isolation and screening of antibacterial and enzyme producing marine actinobacteria to approach probiotics against some pathogenic vibrios in shrimp Litopenaeus vannamei. IJFS. 2016; 15 (2) :630-644
URL: http://jifro.ir/article-1-2199-en.html


Volume 15, Issue 2 (4-2016) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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