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:: Volume 14, Issue 2 (4-2015) ::
IJFS 2015, 14 Back to browse issues page
A new method for venom extraction from venomous fish, Green Scat
S.M Ghafari Khalaf Mohamadi , D Shahbazzadeh , S Jamili , S.M.R Fatemi , K Pooshang Bagheri *
, k_bagheri@pasteur.ac.ir
Abstract:   (3582 Views)
Scatophagus argus argus (Green Scat) is a pretty aquarium fish. Its hard spines are venomous and can cause painful injury. In this study 60 specimens of Green Scat were collected periodically from coastal waters of Boushehr (south of Iran) from May 2011 to April 2012. Anatomical features of venomous spines were investigated. Scat venom was extracted from the spines in a new manner for keeping the specimens alive. The nature of venom was tested by SDS-PAGE. Ethical issues and animal welfare principles such as rapid and instantaneous anesthetizing, post operation disinfection and fast recovery of the specimens was practiced in order to minimize the complications. This method enhanced the purity and quantity of venom as demonstrated by 12 separated proteins in electrophoresis. New ethical issues were developed to surviving the specimens and prolong viability as well.
Keywords: Scatophagus argus argus, Venomous spine, Venom extraction, Viability, Ethical issues, Boushehr
Full-Text [PDF 449 kb]   (1135 Downloads)    
Type of Study: Orginal research papers | Subject: Toxicology
Received: 2015/06/8 | Accepted: 2015/06/8 | Published: 2015/06/8
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Ghafari Khalaf Mohamadi S, Shahbazzadeh D, Jamili S, Fatemi S, Pooshang Bagheri K. A new method for venom extraction from venomous fish, Green Scat. IJFS. 2015; 14 (2) :321-327
URL: http://jifro.ir/article-1-1885-en.html


Volume 14, Issue 2 (4-2015) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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