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:: Volume 13, Issue 2 (4-2014) ::
IJFS 2014, 13 Back to browse issues page
Sequence analysis of ORF94 in different White Spot Syndrome Virus (WSSV) isolates of Iran
M. M Simrouni * , M Mesbah , H Rajabi Memari , M. r Seifi Abad Shapoori , M PurMahdi Boroujeni
, Dr_simrouni@hotmail.com
Abstract:   (4351 Views)
White spot syndrome virus (WSSV) is a pathogen that causes high mortality in shrimp culture in the whole world. Sequence analysis of WSSV has shown similarity of WSSV isolates in different countries with exception of a few variable genomic loci. This study investigated the sequence variation of some Iranian WSSV isolates and previously identified isolates. Samples were collected during target surveillance and were feed, broodstock, post-larvae, artemia, crabs, and wild and cultured shrimp of northern Persian Gulf (Boushehr and Khuzestan provinces). The open reading frame (ORF) 94 sequence of different Iranian WSSV isolates were amplified using specific primers from positive samples. The ORFs 94 sequence of positive samples were sequenced and registered in the Gene Bank and then compared to other WSSV isolates. The number of repeat units in ORF94 showed that WSSV isolates were varied in number. There are SNPs (G and T) in position 48 of RUs that varies in different Boushehr and Khuzestan isolates. Also these sequences were compared to Gene Bank WSSV isolates and showed a high similarity (>90%) to Southeast Asian countries. To our knowledge this is the first report of sequence analysis in Iranian WSSV isolatesapplications
Keywords: WSSV, Sequence analysis, ORF94
Full-Text [PDF 136 kb]   (1900 Downloads)    
Type of Study: Orginal research papers | Subject: fish disease
Received: 2014/06/30 | Accepted: 2014/06/30 | Published: 2014/06/30
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Simrouni M M, Mesbah M, Rajabi Memari H, Seifi Abad Shapoori M R, PurMahdi Boroujeni M. Sequence analysis of ORF94 in different White Spot Syndrome Virus (WSSV) isolates of Iran . IJFS. 2014; 13 (2) :492-502
URL: http://jifro.ir/article-1-1519-en.html

Volume 13, Issue 2 (4-2014) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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