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:: Volume 13, Issue 2 (4-2014) ::
IJFS 2014, 13 Back to browse issues page
Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing
M Mirbakhsh * , A Akhavan sepahy , M Afsharnasab , A Khanafari , M.R Razavi
Abstract:   (4440 Views)
A Gram-negative, rod-shaped, motile and halophilic bacterium identified as Vibrio harveyi was isolated from hatchery-reared larval of Iran Shrimp Research Centerfollowing mass mortality . Seven isolates of Vibrio spp.were collected from nauplii of Litopenaeus vannamei and then were evaluated for characteristics including morphology, physiology and biochemistry. The results indicated that the isolates belonged to a single species that grew on Thiosulfate citrate- Bile Sucrose agar and in 1.5 to 6% NaCl, positive lysine and ornithine decarboxylation and susceptible to O/129 disk. Identification of Vibrio harveyi strainIS01 (1 of 7 isolates) was confirmed by Polymerase Chain Reaction assay for Vibrio harveyi (expected amplicon 1493 bp). The 16S ribosomal DNA sequence (National Center for Biotechnology Information, GenBank accession number GU974342.1) gave 99% sequence identity to V. harveyi ATCC: 35084 (GenBank accession number EU130475.1).
Keywords: Vibriosis, shrimp diseases, Litopenaeus vannamei, 16S rDNA sequencing
Full-Text [PDF 191 kb]   (2066 Downloads)    
Type of Study: Orginal research papers | Subject: Genetics
Received: 2014/06/30 | Accepted: 2014/06/30 | Published: 2014/06/30
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Mirbakhsh M, Akhavan sepahy A, Afsharnasab M, Khanafari A, Razavi M. Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing. IJFS. 2014; 13 (2) :384-393
URL: http://jifro.ir/article-1-1509-en.html


Volume 13, Issue 2 (4-2014) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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