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:: Volume 12, Issue 3 (7-2013) ::
IJFS 2013, 12 Back to browse issues page
A phylogeny analysis on six mullet species (Teleosti: Mugillidae) using PCR-sequencing method
M. Nematzadeh * , S. Rezvani , M.K. Khalesi , F. Laloei , A. Fahim
, nematzadeh.ma@yahoo.com
Abstract:   (7808 Views)
In this study, genetic differences and phylogenic relationships among six Mugilidae species (Mugil cephalus, M. capito, Liza subviridis, L. saliens, L. aurata, Valamugil buchanani) were determined using PCR-sequencing. M. cephalus, L. subviridis, and V. buchanani from the Persian Gulf and Oman Sea, and L. aurata and L. saliens from the Caspian Sea were col-lected. Samples of an imported, Egyptian species M. capito were obtained from the Gomishan Research Center in Gorgan. Total DNA from the samples were extracted according to phenol-chloroform procedure. The extracted total DNAs were amplified using polymerase chain reaction (PCR) and then sequenced. The number of bases in the mitochondrial 16s rRNA genome used in this study approximated 600 base pairs. The size of the bands was identical in all the studied species and no heteroplasmia was observed. In addition, the numbers of variable, preserved, and Pi sites were about 114/624, 488/624, and 110/624, respectively. Analysis of the sequences showed great differences between Mugil species and the other studied species. The phylogenetic tree obtained through Neighbor-Joining method revealed that L. saliens and L. aurata were in the same branch while L. subviridis was in a separate branch. In contrast, Maximum Parsimony tree located L. subviridis and L. aurata in a single branch and assigned L. saliens to a distinct branch. This result brings in the question of monophyletic origin of the genus Liza.
Keywords: Sequencing, Mugillidae, Phylogeny, PCR, Persian Gulf, Oman Sea, Caspian Sea
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Type of Study: Orginal research papers | Subject: aquaculture
Received: 2013/07/28
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Nematzadeh M, Rezvani S, Khalesi M, Laloei F, Fahim A. A phylogeny analysis on six mullet species (Teleosti: Mugillidae) using PCR-sequencing method. IJFS. 2013; 12 (3) :669-679
URL: http://jifro.ir/article-1-1101-en.html


Volume 12, Issue 3 (7-2013) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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