Fungal infection in the eggs of freshwater fish is well known as a problematic disease. The aim of the present study was to isolate and identify parasitic and saprophytic fungi from affected eggs of rainbow trout at two fish hatchery in Kermanshah province. The sample were inoculated in culture media (SDA,CMA,GPA and stilled water with cotton seed culture) at room temperature (18-24 ° C).17 species of fungi isolated from the fungal eggs. Five fungi species that isolated in this study were belonged to the saprolegniaceae family including Saprolegnia paraitica, Saprolegnia lapponica ,Saprolegnia ferax, Saprolegnia hypogyna and Saprolegnia diclina. Another fungi that isolated in this study were, Fusarium oxyparum , Fusarium npoa , Fusarium sp ., Penicilium citrinium, Penicillium expansum, Aspergillus treuse, Aspergillus clavatus, Cladosporium sp., Alternaria sp., Helmintosporium sp., Pscilomyces sp., Mocur. It seems that Saprolegnia parasitica with 26.8 percent of isolation was the most important fungal infestation of egg in kermanshah trout hatcheries. Mocur with 19.6% had the most frequency after S. parasitic and Fusarium, Aspergillus and Pescillomyces with 2.45% had the lowest frequency. In this study S. ferax, S. hypogyna and S. diclina are reported from Iran for the first time.