en Biotechnology Biotechnology پژوهشي Orginal research papers Fungal assist microalgal cultivation is getting substantial considerations due to the highly efficiency of bioflocculation process without demanded of using chemicals and little bit energy input. Therefore, Fungi pelletization-assisted microalgal cultivation were applied for improving algal harvesting process and nutrient recovery as step wise for wastewater treatment. Two strains of marine microalgae (<em>Nannochloropsis salina</em> and <em>Chlorella salina</em>) and three species of marine fungi (<em>Penicillium chrysogenum</em>,<em> Aspergillus niger</em> and <em>Aspergillus fumigatus</em>) were implied to investigate the effect of fungi-algae pelletization process on wastewater recovery. After 48 hour of cultivation, the highest flocculation efficiency (98.9%) was recorded by <em>A. fumigatus</em> assist <em>C. salina</em>, while the lowest percentage (85.9%) recorded by <em>A. niger</em> assist <em>N. Salina</em>. The highest nutrient removal percentage was for nitrite by <em>A. niger</em> assist <em>C. salina</em> /tap water (98.4%) followed by phosphate <em>A. fumigates</em> assist <em>N. salina</em>/sea and tap water (90.9%), then ammonia by <em>A. fumigates</em> assist <em>N. salina</em>/seawater (89.5%). The bioflocculation process is a promising for algal harvesting techniques and wastewater nutrient recovery. Wastewater treatment, Fungi/algae assist, Bioflocculation, Nutrient recovery, Microalgal harvesting 1780 1794 http://jifro.ir/browse.php?a_code=A-10-1799-4&slc_lang=en&sid=1 A.G.M.E. Madkour ag.madkour@niof.sci.eg 100319475328460036103 100319475328460036103 Yes Marine Pollution Department, National Institute of Oceanography and Fisheries, Red Sea, Egypt. H.A.H. Ibrahim drhassanelsharqawy@gmail.com 100319475328460036104 100319475328460036104 No Microbiology Department, National Institute of Oceanography and Fisheries, Alexandria, Egyp W.M.M. El-Sayed : walled_mohamed78@yahoo.com 100319475328460036105 100319475328460036105 No Marine Microbiology Department,National Institute of Oceanography and Fisheries, Red Sea, Egypt K.M. El-Moselhy km.elmoselhy@niof.sci.eg 100319475328460036106 100319475328460036106 No Marine Pollution Department, National Institute of Oceanography and Fisheries, Red Sea, Egypt.