fa fish disease fish disease پژوهشي Orginal research papers <p dir="ltr" style="text-align: justify;">Different modified atmosphere conditions with various CO₂concentrations on microbial load (psychrophile, mesophile aerobic and anaerobic bacteria) and physico-chemical properties of barramundi (<em>Lates &nbsp;calcarifer </em>Bloch) fillets stored at 8&deg;C were compared to determine the best packaging conditions. The gas conditions evaluated were 100% CO₂ (M₁), 75% CO₂/25% N₂(M₂), 50% CO₂/50% N₂ (M₃), 25% CO₂/75% N₂ (M₄) and 100% N₂ (control). High CO₂ concentration (M₁ and M₂) of fish fillets delayed the psychrophilic bacteria growth compared to low CO₂ concentration (50% and 25% CO₂) as well as the mesophilic, anaerobic and histamine forming bacteria. We concluded that the atmosphere with 75:25% and 100:0 (CO₂:N₂) had the most appropriate gas composition to inhibit the microbial growth and prolong the shelf life of barramundi fillets (<em>p<</em>0.05).&nbsp;</p> Barramundi, CO2 concentration, Psychrophile bacteria, Histamine forming bacteria, MAP, Lates calcarifer. 457 469 http://jifro.ir/browse.php?a_code=A-10-1272-176&slc_lang=fa&sid=1 A. Yassoralipour aliyas@utar.edu.my 100319475328460015552 100319475328460015552 Yes J. Bakar 100319475328460015553 100319475328460015553 No R.A. Rahman 100319475328460015554 100319475328460015554 No A. Bakar Fatimah 100319475328460015555 100319475328460015555 No F. Özogul 100319475328460015556 100319475328460015556 No