|
Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing
|
M Mirbakhsh *   , A Akhavan sepahy , M Afsharnasab , A Khanafari , M.R Razavi |
|
|
|
Abstract: (9950 Views) |
| A Gram-negative, rod-shaped, motile and halophilic bacterium identified as Vibrio harveyi was isolated from hatchery-reared larval of Iran Shrimp Research Centerfollowing mass mortality . Seven isolates of Vibrio spp.were collected from nauplii of Litopenaeus vannamei and then were evaluated for characteristics including morphology, physiology and biochemistry. The results indicated that the isolates belonged to a single species that grew on Thiosulfate citrate- Bile Sucrose agar and in 1.5 to 6% NaCl, positive lysine and ornithine decarboxylation and susceptible to O/129 disk. Identification of Vibrio harveyi strainIS01 (1 of 7 isolates) was confirmed by Polymerase Chain Reaction assay for Vibrio harveyi (expected amplicon 1493 bp). The 16S ribosomal DNA sequence (National Center for Biotechnology Information, GenBank accession number GU974342.1) gave 99% sequence identity to V. harveyi ATCC: 35084 (GenBank accession number EU130475.1). |
|
| Keywords: Vibriosis, shrimp diseases, Litopenaeus vannamei, 16S rDNA sequencing |
|
|
Full-Text [PDF 191 kb]
(4558 Downloads)
|
Type of Study: Orginal research papers |
Subject:
Genetics
Received: 2014/06/30 | Accepted: 2014/06/30 | Published: 2014/06/30
|
|
|
|
|
|
|
| Add your comments about this article |
|
|
|
.png)
.png)
.png)
.png)
.png)
